运用PCR-DGGE技术对3种原甲藻18S rDNA部分序列的差异性分析     

孙静  于志刚  甄毓  张涛  赵丽媛  袁健  米铁柱 《中国海洋大学学报(自然科学版)》2010,40(2)

采用3对特异性引物,对中国近海常见3种原甲藻的18S rDNA的部分序列进行PCR扩增,运用变性梯度凝胶电泳技术(Denaturing Gradient Gel Electrophoresis,DGGE)对PCR产物进行了差异性分析。结果表明,各对引物具有不同的区分效果,选择包含较多差异碱基的短片段进行扩增,可以对藻种进行更好的区分;PCR-DGGE技术具有较高的灵敏性,不同藻种间即使1个碱基的差异也可以区分开;PCR-DGGE技术可以对3种原甲藻进行区分。  相似文献   

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry     

HOU Jianjun          LAI Hongyan  HUANG Bangqin  CHEN Jixin College of Fisheries  Huazhong Agricultural University  Wuhan  China College of Life Sciences  Hubei Normal University  Huangshi  China State Key Laboratory of Marine Environmental Science  Environmental Science Research Center  Xiamen University  Xiamen  China Key Laboratory of Freshwater Fish Germplasm Resources  Biotechnology  Yangtse River Fisheries Research Institute  Jinzhou  China State Key La... 《海洋学报(英文版)》2009,28(2)

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes (DNA probes) were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with flu...  相似文献   

流式影像仪在东海海域甲藻藻华研究中的应用     

胡晓坤  赵越  孔凡洲  张清春  严冰  于仁成 《海洋与湖沼》2022,53(2):330-339

有害藻华对海水养殖、人类健康和生态安全构成威胁,已成为全球性的生态灾害.近20年来,米氏凯伦藻(Karenia mikimotoi)、东海原甲藻(Prorocentrum donghaiense)等甲藻形成的有害藻华在东海海域连年暴发,其频率高、危害大,形成及演变机理复杂,对藻华原因种进行监测是开展有害藻华预警和防控的...  相似文献   

Growth inhibition to three red tide microalgae by extracts of Ulva pertusa     

金秋董双林  王长云 《中国海洋湖沼学报》2006,24(2):147-153

1 INTRODUCTION In recent decades, red tides that induced by in- creasing eutrophication occur frequently in coastal areas worldwide. This has caused serious problems in marine environment, aquaculture industries and human health (Qi et al., 1993; Horner e…  相似文献   

Acute Toxicity of Formalin to the Red-tide Dinoflagellate Prorocentrum minimum Schiller(Protozoa,Mastigophora)     

XU Henglong)  )  SONG Weibo)  ZHU Mingzhuang)  QIU Yantao)  and MA Honggang ) The Lab of Protozoology  KLMEM  Ocean University of China  Qingdao  P.R.China ) Department of Biology  Liaocheng University  Liaocheng  P.R.China 《中国海洋大学学报(英文版)》2005,4(1)

1Introduction Red tides occur in recorded history,but in recentyears there is a global increase in the number of theseevents due to coastal pollution and other unclear fac-tors.Some kinds of red tides cause mass deaths of cul-tured animals and thus cause heavy economic losses forcoastwise mariculture(Nakanishi et al.,1996;Changet al.,1998;Zhu et al.,1998;Tada et al.,2001).The investigation on red tide control,however,rem-ains insufficient(Burkholder,1998;Edwards and Clark,1999).Although man…  相似文献   

东海春季赤潮过程中优势种的演替:生物-物理耦合数值模拟   总被引：1，自引：1，他引：0  

孙科  丘仲锋  何宜军  樊伟  魏泽勋 《海洋学报(英文版)》2016,35(12):1-11

In the East China Sea(ECS), the succession of causative species responsible for blooms is a recurrent phenomenon during the spring, which changes from diatoms to dinoflagellates. Observations from space and in situ cruises captured this pattern of succession during spring of 2005. In this study, we coupled two biological models, which were developed previously for Skeletonema costatum and Prorocentrum donghaiense,into a circulation model tailored for the ECS. The coupled biophysical model was used to hindcast the blooms and to test the hypothesis proposed in earlier studies that phosphate(PO4 3–) is the first-order decider of the succession. The coupled model successfully reproduced the hydrodynamics(as described in a companion paper by Sun et al.(1), the spatiotemporal distribution of the chlorophyll a(Chl a) concentration, and the species succession reasonably well. By analyzing the effects of different factors on the surface Chl a distribution, we confirmed that the offshore boundaries of the blooms were confined by PO4 3–. In addition, we suggest that surface wind fields may modulate the horizontal distribution of blooms. Thus, during the dispersal of blooms, surface winds coupled with PO4 3– may control the succession of blooms in the ECS. The proposed coupled model provides a benchmark to facilitate future improvements by including more size classes for organisms, multiple nutrient schemes, and additional processes.  相似文献   

福建闽东海域具齿原甲藻赤潮与生态因子关系的初步分析   总被引：5，自引：2，他引：5  

陈国斌  李奶姜  戴红 《台湾海峡》2003,22(4):431-436

本文根据2002年5月闽东海域一次大面积赤潮的监测数据。对赤潮区赤潮生物——具齿原甲藻密度与生态因子的关系进行初步分析．结果表明：现场水文气象因子对赤潮区具齿原甲藻密度影响不大，但影响赤潮面积的消长；水化因子中对赤潮区具齿原甲藻密度影响明显的因子有化学需氧量、活性磷酸盐．具齿原甲藻密度与化学需氧量、活性磷酸盐的回归方程分别为：Y=1．39X-1．77，Y1．55X-0．56．活性磷酸盐是本次赤潮的主要限制因子之一。  相似文献   

东海原甲藻与塔玛亚历山大藻种群增长过程与种间竞争研究   总被引：4，自引：1，他引：4  

由希华  王宗灵  郝彦菊  李瑞香  朱明远  石晓勇  喻龙 《海洋科学进展》2006,24(4):504-510

针对我国东海2种重要赤潮肇事生物东海原甲藻(Prorocentrum donghaiense)和塔玛亚历山大藻(Alexan-drium tamarense)进行了种群增长过程和种间竞争的实验研究。结果表明,单种培养时,不同氮磷比对东海原甲藻和亚历山大藻种群增长过程没有明显的影响,但是会影响其最终的细胞密度;在混合培养时,营养盐组成比例不同对东海原甲藻和塔玛亚历山大藻的种间竞争过程有影响,但不会影响其最后竞争结果。竞争物种的细胞起始密度对种间竞争结果有明显的影响,P.donghaiense和A.tamarense的接种细胞密度比为2 000∶100和2 000∶200时,东海原甲藻占优势;当接种细胞密度比为4 000∶400时,塔玛亚历山大藻占优势。  相似文献   

氮,磷,维生素和微量金属对赤潮生物海洋原甲藻的增殖效应   总被引：12，自引：1，他引：12  

王正方  张庆 《海洋学研究》1996,14(3):33-38

在室内培养实验中观测了赤潮生物海洋原甲藻ＰｒｏｒｏｃｅｎｔｒｕｍｍｉｃａｎｓＥｈｒｅｎｂ对ＮＯ、ＮＨ和甘氨酸的需求。结果表明，硝酸盐的浓度从４０到３００μｍｏｌ／ｄｍ３，氨盐浓度从５０到１５０μｍｏｌ／ｄｍ３，甘氨酸和谷氨酸混合液浓度从４０到８０μｍｏｌ／ｄｍ３皆能较好地维持海洋原甲藻的增殖。磷酸盐起着磷源作用。在ＥＤＴＡ浓度为８０μｍｏｌ／ｄｍ３的培养液中，Ｆｅ３＋的浓度从０，．５到１μｍｏｌ／ｄｍ３，Ｍｎ２＋从０到２０μｍｏｌ／ｄｍ３和Ｃｏ２＋从０．１到０．４μｍｏｌ／ｄｍ３能促使海洋原甲藻的增殖。维生素效应试验表明：维生素Ｂ１是生长促进因子，而维生素Ｂ１２和生物素在光和暗处皆未能促进海洋原甲藻的增殖。无机氮和磷的最小细胞额分别为０．７４×１０－１２ｍｏｌ／细胞和０．０４５×１０－１２ｍｏｌ／细胞，其中磷比氮更能限制海洋原甲藻的增殖。  相似文献   

Bioavailability of dissolved organic phosphorus compounds to typical harmful dinoflagellate Prorocentrum donghaiense Lu   总被引：4，自引：0，他引：4  

Huang B  Ou L  Hong H  Luo H  Wang D 《Marine pollution bulletin》2005,51(8-12):838-844

The bioavailability of dissolved organic phosphorus (DOP) compounds to harmful alga Prorocentrum donghaiense Lu and its alkaline phosphatase activity (APA) were studied. Results showed that P. donghaiense could utilize the test DOP compounds such as glucose-6-phosphate (G-6-P), adenosine triphosphate (ATP) and ribonucleic acid (RNA) to sustain its growth. Nutrition between the test DOP compounds and orthophosphate was comparable. P. donghaiense could utilize intracellular phosphorus to sustain growth under depletion of dissolved phosphorus. Variation of APA in different test DOP compounds was addressed; the controlling mechanism of APA in different DOP media was discussed.  相似文献